The Mouse Oral Microbiome Database (MOMD) is a curated online resource for for mouse-based studies. It is constructed by a combination of bacterial culture, 16S rRNA gene amplicon and whole genome sequencing. The taxonomy is based on full length 16S rRNA sequences from cultured isolates as well as draft genomes from representative taxa, collected from sources including laboratory mice, wild Mus musculus domesticus and wild wood mice Apodemus sylvaticus. At present it comprises 103 Mouse Oral Taxa (MOT) spanning four phyla – Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes including 12 novel, previously undescribed species-level taxa. The current MOMD indicates a low diversity & predominantly culturable nature of the laboratory mouse oral microbiome and identifies three major murine-specific oral bacterial lineages, namely Streptococcus danieliae (MOT10), Lactobacillus murinus (MOT93) and Gemella species 2 (MOT43).

MOMD aims to serve as a useful resource for in vitro experimentation and mouse model studies in the field of oral microbiology. MOMD provides tools including a reference set of mouse oral microbial 16S rRNA gene sequences, a BLASTN search tool for 16S rRNA gene identificaiton, online search and browsing tools for genome annotations, as well as BLAST search against genomic contigs and annotations.

MOMD Tools and resource:

  1. Browse, Search and Download MOMD Taxonomy
  2. BLASTN Search against MOMD 16S rRNA Reference Sequences
  3. Download MOMD 16S rRNA Reference Sequences
  4. MOMD Genome Annotaion and Browser
  5. BLAST Search against MOMD Genomes
  6. Download MOMD Data

Investigators

Tsute Chen, Susan Joseph, George Weinstock, Floyd E. Dewhirst, William G. Wade, and Michael A. Curtis

Contributors

Joseph Aduse-Opoku, Ahmed Hashim, Eveliina Hanski, Ricarda Streich, Sarah C. L. Knowles, and Amy B. Pedersen

Funding

The compilaton of the current MOMD 16S rRNA reference sequences was funded by the UKRI Medical Research Council (award MR/P012175/2). The wild Mus musculus sequence isolation work was funded by a NERC fellowship NEL011867/1 to S.C.L.K.
MOMD is funded by Grant 2R01DE016937-16 "A Foundation for the Oral Microbiome and Metagenome" from The National Institute of Dental and Craniofacial Research.



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Page last updated: July 7, 2021 08:39:35
 
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